We’ve obtained, produced and characterized, American mink iPS cells. These cell lines contain diploid chromosome numbers, and are pluripotent comfirmed by the performed teratoma formation test.
The majority of the analyzed cell lines contained a normal number of diploid chromosome . The pluripotency of iPS cell lines was confirmed by formation of teratomas with cell types representing all three germ layers. Transcriptome analysis of mink embryonic fibroblasts (EF) and two iPS cell lines allowed us to identify a certain number of assembled contigs which were annotated. These led to a certain number unique genes. We analyzed the expression levels of these genes in the iPS cell lines. This comfirmed that 80% of genes were correctly reprogrammed in iPS cells. The expression of pluripotency marker genes such as Oct4, Sox2 and Rex1 in iPS cell lines with notable exception of Nanog was confirmed.
Conclusively it can be confirmed that the production and characterization of American mink iPS cells was achieved. These cells were pluripotent by a number of criteria and the iPS cells exhibited effective reprogramming.
iPS cell examples of cell types in teratomas developed from mink iPS cells, here an epidermal epithelium with hair follicles.
The MINK iPSc’ are stored in liquid nitrogen and will be used as the basic cell-lines for our scale up production of FUROID ™️ prototypes.