FIG. 1: illustrates pattern printing in cells. Attached are pictures of light-mediated gene activation by blue light that activates cell states. It’s visible that very small patterns, such as polka dots or a smile face are printed on the cells.


FUROID ™️ invented a process of cell specific enrichment, genetic engineering, differentiation and disposition of specific cells in engineered tissues. It relates to pre-coding and to the use of a combination of genetic signature combinations as an anti-counterfeit mechanism and as proof of authenticity, timestamp and provenance.

 The attached drawing * FIG . 3 illustrates a anti-counterfeit genetic signature combination intrinsically integrated in the bioengineered tissue, derived product, product batch, or product types, and includes as well a timestamp. The genetic signature is inseparably connected to the bioengineered tissue and its derived products.

Furthermore, the bioengineered tissue will be inseparably attached to a combination of nucleic acid molecular signature combinations that allows determining its authenticity, and assist the manufacturing process by enabling genetically encoded batch labels, product type labels, genetic time-stamping, proof of provenance and anti-counterfeit measures whilst the manufacturing process.

The use of “barcode” refers to a nucleic acid sequence of at least one nucleotide and a barcode presents 4^n combinations where n is the arbitrary length of said barcode and correspond to the main nucleic acid bases present in vertebrata produced or grown tissues and reducing the epigenetic modifications or chemical modifications to its principal purine or pyrimidine core.

A nucleic acid molecule containing at minimum one nucleic acid barcode, and comprising at minimum one nucleotide sequence encoding a selection marker indicating homologous or heterologous recombination when integrated in the genome of a vertebrata cell, wherein the selection markers when being expressed are optically discriminable, e.g. in FACS or any fluorescence guided capture, and wherein the nucleotide sequence encoding a selection marker indicating homologous or heterologous recombination in a vertebrata cell is flanked 5' and/or 3' by nucleotide sequences that are homologous to nucleotide sequences of a nucleic acid sequence present in the vertebrata cell.

A mean and method for determining the authenticity of the bioengineered tissue and its derivative products could be described partially as follows:

  1. The bioengineered tissue carries an inseparable molecular signature or molecular fingerprint composed by the combination of combinations of nucleic acid barcodes held within the genome of cells.

  2. Part of the bioengineered tissue and holder of the properties can be analyzed by DNA sequencing to detect the presence, composition and dose of the nucleic acid barcodes.

  3. The readout constitutes a binary-readout of the authenticity of the bioengineered tissue and its derivative products. The readout enables demonstrating the source, production batch, production time and excluding counterfeit or adulteration of the item its derived products and items irreversibly attached to item.

  4. The properties, the analysis and readout of it constitutes a mean of authenticity and a mechanism of anti-counterfeit, proof of provenance and allow differentiating item from other tissues derived from farming or poaching.

Conclusions:

The fibroblast-like cell, interfollicular epidermis cell, and other cells, are genetically engineered so as to carry one or more characteristic and different DNA sequences integrated into their genome in a way that generate a unique combination of molecular signatures.

The combination of molecular signatures constitutes a proof of authenticity and a mechanism of anti-counterfeit as it is designed by and known to the manufacturer, and can be assigned at will to production batches of bioengineered tissues, and or product-derivative types containing bioengineered tissues. Which will be stored in the blockchain * FIG. 2.

As a fourth labeling and security feature we will use BLOCKCHAIN TECHNOLOGY in order to provide security to clients and end users that the purchased FUROID™️ materials are 100% cruelty free obtained invitro fur and wool.

The combination of molecular signatures can in turn be analyzed by DNA sequencing technologies and said result used as proof of authenticity and provenance.



FIG. 2: Applications examples with Blockchain technology.


FIG.3: illustrates a anti-counterfeit genetic signature combination intrinsically integrated in the bioengineered pelt, derived product, product batch, product types and timestamp. The genetic signature is inseparably connected to the bioengineered pelt and its derived products.